Figure 2.

CD3 aptamer binding characterization in vitro

(A) Binding of P32 radiolabeled CD3 aptamers to rmCD3 target protein and hIgG1 control protein in a nitrocellulose binding assay. CD3 candidate aptamers were radioactively labeled, and co-incubated with serially diluted CD3 control protein or hIgG1 target protein. Aptamers 1, 3, 5, and 12 were determined to be specific binders to the target protein rmCD3. (B) Aptamers 1, 3, 5, and 12 bind to rmCD3 target protein as determined by surface plasmon resonance. (C) Binding of fluorescently labeled CD3 aptamers to recombinant murine-CD3δε target protein-coated beads. (D) Binding of fluorescently labeled CD3 aptamers to recombinant human-CD3δε target protein-coated beads and not to human IgG1 nor to murine-CD3γε target protein-coated beads.