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. 2024 Apr 23;35(2):102198. doi: 10.1016/j.omtn.2024.102198

Figure 3.

Figure 3

CD3 aptamer binding to CD3-expressing cells

(A) Binding of CD3 aptamers to wild-type EL-4 cells and different CD3 chain KO cells. (B) Quantitative representation of the specificity of binding of CD3 aptamers to CD3+ WT EL-4 cells as compared with CD3δKO, EL-4 CD3εKO, and EL-4 CD3γKO cell lines. We calculated the percentage of reduction in stain index of each of the aptamers in each of the CD3KO cell lines, as compared with their stain index in CD3+ WT cells. All stain indexes were normalized to the signal of the control aptamer. A reduction in stain index in the CD3KO cell line compared with the WT cell line is indicative of specific binding (mean ± SEM). n = 2–3. Two-way ANOVA was performed as compared with the basal stain index. ∗∗∗p < 0.001, ∗∗p < 0.01, ∗p < 0.05. Anti-CD3 antibodies 17A2 and 2C11 were used as positive control.