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Ras-GAP complexed to ICP10 in HSV-2-infected cells is phosphorylated. (A) Immunocomplex PK assays of extracts from cells mock infected (lane 1) or infected with HSV-2 at 2 h (lane 2), 4 h (lane 3), 8 h (lane 4), or 12 h (lane 5) p.i.; ICP10ΔPK at 2 h (lane 6), 4 h (lane 7), 8 h (lane 8), or 12 h (lane 9) p.i.; or HSV-2(R) at 4 h (lane 10) p.i. (B) Immunoblotting of gel in panel A with antibody to ICP10. (C) Immunoblotting of gel in panel A with antibody to Ras-GAP.
