FIG. 7.

GTPase activity is lower in HSV-2-infected cells than in ICP10ΔPK-infected cells. Extracts of cells infected with HSV-2 (□), HSV-2(R) (▴), or ICP10ΔPK (●) or mock infected with PBS in 1% FCS (▵) for 4 h were immunoprecipitated with anti RAS antibody (F123-62). GTPase reactions were performed on the immunoprecipitates with [α-³²P]GTP and chromatographed on PEI plates. Data represent the percent GTP hydrolyzed at each time point ± the standard error of the mean. Similar results were obtained in ICP10ΔPK- and mock-infected cells in 10% FCS.