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. 2000 Nov;74(22):10417–10429. doi: 10.1128/jvi.74.22.10417-10429.2000

FIG. 8.

FIG. 8

MAPK is activated in HSV-2-infected cells but not ICP10ΔPK-infected cells. Vero cells were mock infected with PBS in 1% (lane 1) or 10% (lane 2) FCS or were infected with HSV-2 for 2 h (lane 3), 4 h (lane 4), 10 h (lane 5), or 16 h (lane 6); HSV-2 in the presence of 50 μM PD98059 for 4 h (lane 7); or ICP10ΔPK for 2 h (lane 8), 4 h (lane 9), 10 h (lane 10), or 16 h (lane 11). Extracts obtained at these times were immunoblotted with antibody to the phosphorylated MAPK (P-MAPK; top panel), stripped, and reblotted with antibody to MAPK1/2 (bottom panel).