. 2000 Nov;74(22):10417–10429. doi: 10.1128/jvi.74.22.10417-10429.2000

TABLE 1.

ICP10 mutants used in binding assays^a

Protein	ICP10 position	PK activity
ICP10	Wild type	+ (Ser-Thr)
p139^TM	Δ86–105	−
p140^l^y^s	Lys¹⁷⁶ and Lys²⁵⁹	−
p140^III	Glu²⁰⁹	−
p140^p^r^o	Pro-rich^{150 and 395}	+ (Ser-Thr)
p136^I/II	Δ106–178	+ (Ser-Thr)
pp29^la1	1–283	+(Thr)

The construction and properties of the ICP10 and mutant eukaryotic expression vectors and the establishment of constitutively expressing cell lines were described earlier (49, 50, 60). p139^TM is deleted in the transmembrane domain (50). p140^lys is mutated in Lys¹⁷⁶ and Lys²⁵⁹, p140^III is mutated in Glu²⁰⁹, and p140^pro is mutated in SH3 binding sites at positions 150 and 395 (60). p136^I/II is deleted in amino acids 106 to 178 (50). pp29^la1 is expressed by the bacterial vector pJL11 (49).