Fig. 2.

5-HT2bR antagonist activity of MW071. MW071 exhibits a concentration dependent antagonist activity with an IC₅₀ = 54 nM. The assay was done by use of a stably transfected CHO cell line and monitoring dose dependent calcium flux using FLIPR technology [15]. All sample plates were subjected to baseline corrections and maximum fluorescence values were used to calculate percentage activation and percentage inhibition according to the equation of (Max RLU) - (Baseline Avg.) / (Positive Avg.) - (Baseline Avg.). Concentrations of MW071 used in the assay for antagonist activity range was 40,000 nM to 2.4 nM.