Fig. 3. Characterization of the secreted recombinant HeLac4c enzyme.

Identification of protein N-glycosylation sites and copper binding sites of laccases expressed in S. cerevisiae (A). SDS-PAGE stained with Coomassie Brilliant Blue R-250 of HeLac4c with and without Endo H treatment for the removal of N-glycosylation (B). Effect of pH on oxidation activities of recombinant laccase (C) and stability of HeLac4c after pretreatment for 2 h at 10−80°C (D) Activity assays of recombinant HeLac4c enzyme were performed by oxidation of ABTS in 100 mM citrate-phosphate buffer at pH 4.0.