Table 1.
Primary antibodies used in this study
Antibody | Type | MW | Specificity | Source | Catalog # |
CAMK II | Mouse | 50KD | CaMKII | Santa Cruz | sc-5306 |
CAMK II α-Subunit | Mouse | 50KD | p-CaMKII (Thr286) | Calbiochem | NB13 |
GSK-3β | Rabbit | 46KD | GSK-3β | Cell signaling | 27c10 |
P-GSK-3β | Rabbit | 46KD | p-GSK-3β (S9) | Cell signaling | 9336 |
MAPK (Erk1/2) | Rabbit | 42/44 | Total p44/42 MAP Kinase (Erk1/Erk2) | Cell signaling | 9102 |
P-MAPK (Erk1/2) (197G2) | Rabbit | 42/44 | p-p44/p42 MAP Kinase (Thr202/Tyr204) | Cell signaling | 4377 |
Cdk5 (DC17) | Mouse | 34 | Cdk5 | Santa Cruz | sc-249 |
P-Cdk5 (C7) | Mouse | 34/37 | p-Cdk5 (Ser159) | Santa Cruz | sc-377558 |
p35 (4G11) | Mouse | 35 | Cdk5 regulatory subunit, p35 | Santa Cruz | sc-293184 |
AMPK | Rabbit | 62 | Total AMPK | Cell signaling | 2532 |
P-AMPK (40H9) | Rabbit | 62 | p-AMPK (Thr172) | Cell signaling | 2535 |
AKT (pan) (11E7) | Rabbit | 60 | Total AKT | Cell signaling | 4685 |
P-AKT (193H12) | Rabbit | 60 | p-AKT (Ser473) | Cell signaling | 4058 |
JNK2 (56G8) | Rabbit | 46/54 | Total JNK2 | Cell signaling | 9258 |
P-SAPK/JNK | Rabbit | 46/55 | p-JNK (Thr183/Tyr185) | Cell signaling | 9251 |
P13 Kinase p85 | Rabbit | 85 | P13 Kinase p85 not p50 or p55 | Cell signaling | 4292 |
P-P13 kinase p85 | Rabbit | 85 | p-p85/p55 (Tyr458/TYr199) | Cell signaling | 4228 |
PDK | Rabbit | 58/68 | Total PDK | Cell signaling | 3062 |
P-PDK | Rabbit | 58/69 | p-PDK (S241) | Cell signaling | 3061 |
mTOR (7C10) | Rabbit | 289 | Total mTOR | Cell signaling | 2983 |
P-mTOR (D9C2) | Rabbit | 289 | p-mTOR (Ser2448) | Cell signaling | 5536 |
PP2A (46/PP2A catalytic α) | Mouse | 36 | PP2Ac | BD Trans. Lab | 610555 |
Demethylated PP2A-C (4i57) | Mouse | 36 | Unmethylated PP2Ac subunit | Santa Cruz | sc-80990 |
Synpsin-1 | Rabbit | 80 | Synpsin-1 | Stressgen | VAP-SV060 |
PSD 95 | Rabbit | 95 | PSD 95 | Cell signaling | 3450 |
NMDA-R2B | Rabbit | 190 | NMDA-R2B | Cell signaling | 4207 |
R92e | Rabbit | 50–75 | pan tau | Pei et al. (1998) | |
anti pS199 | Rabbit | 50–75 | p-tau (S199) | Invitrogen | 44734G |
anti-pT205 | Rabbit | 50–75 | p-tau (T205) | Invitrogen | 44738G |
anti-pT212 | Rabbit | 50–75 | p-tau (T212) | Invitrogen | 44740G |
anti-pS214 | Rabbit | 50–75 | p-tau (S214) | Invitrogen | 44742G |
anti-p262 | Rabbit | 50–75 | p-tau (S262) | Invitrogen | 44750G |
PHF-1 | Mouse | 50–75 | p-tau (S396/404) | Dr. P. Davis | |
anti-PS409 | Rabbit | 50–75 | p-tau (S409) | Biosource | 44-760G |
GAPDH | Rabbit | 37 | GAPDH | Sigma | G9545 |