FIG. 5.

(A) Effects of BDV acute infection on FeAst glutamate uptake. At the indicated time points after infection with BDV, the ³H-Glu uptake assay was performed, as described in Materials and Methods. Results are expressed as the ratio of ³H-Glu uptake of infected cultures over that of control cultures. (B) Effects of BDV persistent infection on FeAst glutamate uptake. At the indicated time points after infection with BDV, the ³H-Glu uptake assay was performed, as described in Materials and Methods. Results are expressed as the ratio of ³H-Glu uptake of infected cultures over the value of the control cultures ± standard error of the mean. Results were analyzed by analysis of variance. ∗, P < 0.05; ∗∗, P < 0.01; ∗∗∗, P < 0.001. (C) Effects of BDV persistent infection on astrocyte glucose uptake. At the indicated time points with BDV, the ability of the feline astrocyte cultures to remove ³H-2DG from the culture supernatant was determined, as described in Materials and Methods. Results are expressed as the ratio of [³H]glucose uptake of infected cultures over the value of the control cultures.