FIG. 4.

Cytokine transcription in PBMCs of cats in the two control groups (◊, gold-immunized cats; ■, gp140-immunized cats). Transcription of (A) IL-10, (B) IFN-γ, (C) IL-12 p35, and (D) IL-12 p40 was measured with quantitative real-time TaqMan PCR. PBMCs were collected at each date of immunization, at challenge, and at weeks 2, 4, 8, 10, and 12. Each cytokine signal is normalized against the GAPDH signal and then calibrated against the preimmune normalized cytokine signal. Bars represent mean normalized cytokine signals for four cats per control group. For standard deviations at week 8, see Fig. 6. I, immunization; 3d I+, third immunization plus 2 days; wk, week.