Table 3.
Primers used in this study for detection of Glu-1By alleles
| PS | Gene | Detection region | Marker name | Type of primer | sequence (5’-3’) | AT | Agarose gel (%) | Reference | |
|---|---|---|---|---|---|---|---|---|---|
| i-MAX II | Ex Taq | ||||||||
| PS15 | Glu-1By8 | CDS | ZSBy8F5 | SNP (By8 dominant) | TTAGCGCTAAGTGCCGTCT | 69℃ | 68℃ | 1% | [24] |
| ZSBy8R5 | TTGTCCTATTTGCTGCCCTT | ||||||||
| PS16 | Multi-gene | CDS | By18-SNP-F | SNP (By8 negative) | TTAGCGCTAAGTGCCGTCC | 66℃ | 66℃ | 1% | [24] |
| By18-SNP-R | TTGTCCTATTTGCTGCCCTT | ||||||||
| PS17 | Glu-1By18 | CDS | MHBy18-F | SNP | CAACTTCTCCACAACAGTCG | 68℃ | 69℃ | 1% | this study |
| MHBy18-R | GTCCTAGTTGGTGTCCTTGTT | ||||||||
| PS18 | Gu-1By9/15/20 | CDS | By01-F | Indel (-45 bp) | GGGGCCATATTATCCAGGCC | 66℃ | 68℃ | 1% | [37] |
| By02-R | TTTGTCCTAGCTGTCCTATTTGT | ||||||||
| PS19 | Gu-1By9/15/20 | CDS | ZSBy9aF1 | Indel (-45 bp) | TTCTCTGCATCAGTCAGGA | 60℃ | 66℃ | 1% | [24] |
| ZSBy9aR3 | AGAGAAGCTGTGTAATGCC | ||||||||
| PS20 | Gu-1By9/15/20 | CDS | MHBy9-F2 | Indel | GGGCAGCAAATAGGACAAGA | ND | 66℃ | 1% | this study |
| MHBy9-R | TTTGTCCTAGCTGTCCTATTTGT | ||||||||
| PS21 | Gu-1By9/15/20 | CDS | MHBy1520-F | Indel | TCTCCACAGCAGCTAGGACG | 66℃ | 66℃ | 1% | this study |
| MHBy1520-R | GCTGTTGAGAAGCTGGGTAC | ||||||||
| PS22 | Glu-1By16 | CDS | ZSBy9F2 | Indel | GCAGTACCCAGCTTCTCAA | 68℃ | 66℃ | 1% | [24] |
| ZSBy9R2 | CCTTGTCTTGTTTGTTGCC | ||||||||
| PS23 | Glu-1By16 | CDS | MHBy16-F | SNP | GGGCAGCAAATAGGACAAGA | 68℃ | 68℃ | 1% | this study |
| MHBy16-R | TTGTCCTACCTGCTGCAGG | ||||||||
PS Primer set, CDS Coding sequence, ND Not determined, AT Annealing temperature, new newly developed marker