FIG. 2.

Methylation status of Cp and Wp regulatory regions in EBV-positive cells. (A) Diagram showing the main regulatory elements of Cp and Wp and the relative positions of CpG dinucleotides analyzed. (B, C, and D) Results of bisulfite sequencing of these regions in BL biopsy specimens (B), in BL cell lines (C), and in long-established LCLs (D). Several clones of the relevant PCR products were sequenced for each cell sample, and the individual CpG dinucleotides were identified as either methylated (+, shaded) or nonmethylated (−); the overall percentage of methylated CpGs per sample is shown.