TABLE 1.
Oligonucleotide primers used for bisulfite genomic sequencing analysis
| Primer | Sequence | Coordinatesa |
|---|---|---|
| Cp 5′ outer | 5′-AGGGCGGCCTTGCGAACAATTATTAGTAGC | 10731–10760 |
| Cp 3′ outer | 5′-ACCACTTTAGACGTTGCTCCACCTCTAAGG | 11157–11128 |
| Cp modified outer 5′ | 5′-AGGGTGGTTTTGTGAATAATTATTAGTAGT | 10731–10760 |
| Cp modified outer 3′ | 5′-ACCACTTTAAACATTACTCCACCTCTAAAA | 11157–11128 |
| Wp 5′ outer | 5′-AAAGCGGGTGCAGTAACAGGTAATCTCTGG | 13971–14000 |
| Wp 3′ outer | 5′-CTCCTGGCGCTCTGATGCGACCAGAAATAG | 14399–14370 |
| Wp modified outer 5′ | 5′-AAAGTGGGTGTAGTAATAGGTAATTTTTGG | 13971–14000 |
| Wp modified outer 3′ | 5′-CTCCTAACACTCTAATACAACCAAAAATAA | 14399–14370 |
| Cp 5′ inner | 5′-AATGTGTCCCAATTAGAAACCC | 10839–10861 |
| Cp 3′ inner | 5′-TTGAGCTCTCTTATTGGCTATA | 11091–11070 |
| Cp modified inner 5′ | 5′-AATGTGTTTTAATTAGAAATTT | 10839–10861 |
| Cp modified inner 3′ | 5′-TTAAACTCTCTTATTAACTATA | 11091–11070 |
| Wp 5′ inner | 5′-GACACTTTAGAGCTCTGGAGGAC | 14023–14045 |
| Wp 3′ inner | 5′-CCCTCCCTAGAACTGACAATTGGC | 14335–14312 |
| Wp modified inner 5′ | 5′-GATATTTTAGAGTTTTGGAGGAT | 14023–14045 |
| Wp modified inner 3′ | 5′-CCCTCCCTAAAACTAACAATTAAC | 14335–14312 |
a
Genome coordinates are given with reference to the B95.8 genomic sequence (6).