Fig. 9.

Histological analysis—Hippocampus. Histological staining results are depicted in representative images per group per staining for the hippocampus (HIP) in all groups. a H&E staining between groups as overview (Scale bar 3 mm) and magnification (Scale bar 150 µm). The black rectangular depicts magnification area. b No difference in NeuN⁺ cells between groups. c Aβ_1-42 positive plaques were visible in APPPS1 animals (Scale bar 150 µm). d Microglia staining using Iba-1. Ramified/resting phenotype in WT brains and activated amoeboid phenotype of microglia in transgenic AD animals was observed. (Scale bar 150 µm). e GFAP⁺ cells in HIP of APPPS1 animals and WT-WD. (Scale bar 150 µm). f CD3⁺ T cells observed mainly in APPPS1 hippocampi (Scale bar 100 µm). g Mean ± SD Aβ_1-42 positive plaques load in HIP in transgenic mice. h Mean ± SD Iba-1⁺ cells were elevated in APPPS1 animals. i Mean ± SD GFAP⁺ cells elevated in WT-WD and in APPPS1 mice. j S Higher mean ± SD CD3 positive T cells in APPPS1-WD group compared to the other groups. HIP = hippocampus. WT-ND n = 2; APPPS1-ND n = 3; WT-WD n = 3; APPPS1-WD n = 3