. 2024 May 14;21:129. doi: 10.1186/s12974-024-03080-0

Table 2.

Two-way ANOVA results showing degree of freedom (Df), F value (F), and significance (p-value) for the flow cytometry data divided into factors ‘genotype’ and ‘diet’

	Factor	Df	F	p-value		factor	Df	F	p-value
a	Brain myeloid cells				b	Brain T cells
CD11b⁺cells	Genotype	1	3.095	0.088	CD3⁺cells	Genotype	1	2.304	0.138
CD11b⁺cells	Diet	1	0.435	0.514	CD3⁺cells	Diet	1	5.279	0.028*
CD1b⁺F4/80⁺cells	Genotype	1	1.526	0.225	CD8⁻cells	Genotype	1	0.478	0.494
CD1b⁺F4/80⁺cells	Diet	1	0.882	0.354	CD8⁻cells	Diet	1	6.554	0.015*
CD11b⁺Ly6G⁺cells	Genotype	1	0.016	0.898	CD8⁺cells	Genotype	1	6.360	0.017*
CD11b⁺Ly6G⁺cells	Diet	1	16.710	< 0.001***	CD8⁺cells	Diet	1	3.735	0.062#
CD11c⁺MHCII⁺DCs	Genotype	1	0.355	0.555	Tregs	Genotype	1	0.915	0.346
CD11c⁺MHCII⁺DCs	DIET	1	5.853	0.021*	Tregs	Diet	1	3.640	0.065#
c	Brain CD8⁻ T cell				d	Brain CD8⁺ T cell
CD62L⁺CD44⁺T_CM	Genotype	1	0.025	0.876	CD62L⁺CD44⁺T_CM	Genotype	1	1.569	0.219
CD62L⁺CD44⁺T_CM	Diet	1	5.279	0.028*	CD62L⁺CD44⁺T_CM	Diet	1	1.647	0.208
CD62L⁻CD44⁺T_EM	Genotype	1	0.225	0.639	CD62L⁻CD44⁺T_EM	Genotype	1	5.910	0.021*
CD62L⁻CD44⁺T_EM	Diet	1	55.500	< 0.001***	CD62L⁻CD44⁺T_EM	Diet	1	3.071	0.089
CD62L⁺CD44⁻naive	Genotype	1	4.987	0.032*	CD62L⁺CD44⁻naive	Genotype	1	4.387	0.044*
CD62L⁺CD44⁻naive	Diet	1	2.337	0.136	CD62L⁺CD44⁻naive	Diet	1	0.079	0.781
CD69⁺CD44⁺effector	Genotype	1	0.007	0.935	CD69⁺CD44⁺effector	Genotype	1	5.622	0.024*
CD69⁺CD44⁺effector	Diet	1	14.400	< 0.001***	CD69⁺CD44⁺effector	Diet	1	1.922	0.175
PD1⁺	Genotype	1	0.036	0.851	PD1⁺	Genotype	1	4.426	0.043*
PD1⁺	Diet	1	2.939	0.096	PD1⁺	Diet	1	0.320	0.575
e	WAT T cells				f	WAT myeloid cells
CD3⁺cells	Genotype	1	0.067	0.798	CD11b⁺cells	Genotype	1	1.944	0.173
CD3⁺cells	Diet	1	4.090	0.052#	CD11b⁺cells	diet	1	7.339	0.011*
CD8⁻cells	Genotype	1	0.136	0.715	CD11b⁺F4/80⁺cells	Genotype	1	1.003	0.324
CD8⁻cells	Diet	1	8.452	0.007**	CD11b⁺F4/80⁺cells	Diet	1	36.400	< 0.001***
CD8⁺cells	Genotype	1	0.005	0.944	M1 macrophages	Genotype	1	0.993	0.326
CD8⁺cells	Diet	1	0.031	0.861	M1 macrophages	Diet	1	52.810	< 0.001***
Tregs	Genotype	1	0.00	0.990	CD11c⁺MHCII⁺F4/80⁻DCs	Genotype	1	2.505	0.123
Tregs	Diet	1	9.480	0.004**	CD11c⁺MHCII⁺F4/80⁻DCs	Diet	1	13.370	< 0.001***

The results are presented: (a) brain myeloid cells, (b) brain T cells, (c) brain CD8- T cell subpopulations, (d) brain CD8 + T cell subpopulations, (e) WAT T cells and (f) WAT myeloid cells

DC, dendritic cells; T_CM, central memory T cells; T_EM, effector memory T cells; Tregs, regulatory T cells

*p < 0.05, **p < 0.01, ***p < 0.001