| Antibodies |
| PGT145 |
NIH AIDS Reagent Program |
Cat# bNAberID_37; RRID:AB_2491054 |
| CAP256-VRC26.25 |
NIH/VRC |
N/A |
| Germline VRC26 |
This study |
N/A |
| Anti-mouse CD180 |
BD Biosciences |
Cat# 562191; RRID:AB_11153851 |
| Goat anti-Mouse IgG-HRP (Fab specific) |
Sigma-Aldrich |
Cat# A9917; RRID:AB_258476 |
| Goat anti-Human IgG-HRP (Fc specific) |
Sigma-Aldrich |
Cat# A0170; RRID:AB_257868 |
| FITC anti-mouse IgM |
Miltenyi Biotec |
Cat# 130-095-906;RRID:AB_10829949 |
| PerCP/Cyanine5.5 anti-CD19 |
BioLegend |
Cat# 152405; RRID:AB_2629814 |
| FITC anti-CD45.1 |
BioLegend |
Cat# 110706; RRID:AB_313495 |
| APC/Cyanine7 anti-CD38 |
BioLegend |
Cat# 102727; RRID: AB_2616967 |
| PE anti-GL7 |
BioLegend |
Cat# 144607; RRID: RRID:AB_2562925 |
| Biotin anti-CD45.2 |
BioLegend |
Cat# 109803; RRID:AB_313440 |
| Bacterial and virus strains |
|
|
| NEB Stable Competent E. coli
|
NEB |
Cat# C3040H |
| NEB 5-alpha Competent E. coli
|
NEB |
Cat# C2987U |
| Chemicals, peptides, and recombinant proteins |
|
|
| NH4Cl Lysing Buffer |
BD Biosciences |
Cat# 555899 |
| Non-Essential Amino Acids |
Thermo Fisher Scientific |
Cat# 11140050 |
| Sodium pyruvate |
Thermo Fisher Scientific |
Cat# 11360070 |
| HEPES |
Thermo Fisher Scientific |
Cat# 15630080 |
| 2-Mercaptoethanol |
Thermo Fisher Scientific |
Cat# 21985023 |
| Penicillin and streptomycin |
Thermo Fisher Scientific |
Cat# 15140163 |
| LPS |
Sigma-Aldrich |
Cat# L2880 |
| Mouse IL-4 |
PeproTech Inc |
Cat# 214-14 |
| Mb2Cas12a |
Ou et al., 2021 |
N/A |
| PBS with Ca2+/Mg2+
|
Thermo Fisher Scientific |
Cat# 14040133 |
| MgCl2 elution solution |
Thermo Fisher Scientific |
Cat# 21027 |
| DAPI |
BioLegend |
Cat# 422801 |
| CleanCap Reagent AG |
TriLink |
Cat# N-7413 |
| m1-pseudouridine-5’-triphosphate |
TriLink |
Cat# N-1081 |
| Cellulose |
Sigma-Aldrich |
Cat# C6288 |
| SM-102 |
MedChemExpress |
Cat# HY-134541 |
| DSPC |
Avanti |
Cat# 850365 |
| Cholesterol |
Sigma-Aldrich |
Cat# C8667 |
| PEG2000 PE |
Avanti |
Cat# 880150 |
| MPLA |
Invivogen |
Cat# vac-mpls |
| Quil-A |
Invivogen |
Cat# vac-quil |
| TMB-ELISA substrate |
Thermo Scientific |
Cat# 34028 |
| TMB Stop solution |
SouthernBiotech |
Cat# 0412-01 |
| Critical commercial assays |
|
|
| Quant-iT RiboGreen RNA Assay Kit |
Thermo Fisher Scientific |
Cat# R11490
|
| Britelite Plus Reporter Gene Assay System |
PerkinElmer |
Cat# 6066761 |
| Deposited data |
|
|
| Mouse IgH library |
This paper; deposited to NCBI SRA |
SRA12195590 |
| Experimental models: Cell lines |
|
|
| Expi293F cells |
Thermo Fisher Scientific |
Cat# A14527 |
| GnTI- Expi293F cells |
Thermo Fisher Scientific |
Cat# A39240 |
| TZM-bl cells |
NIH AIDS Reagent Program |
Cat# 8129 |
| HEK293T cells |
ATCC |
Cat# CRL-3216 |
| Experimental models: Organisms/strains |
|
|
| Mouse: C57BL/6J |
The Jackson Laboratory |
JAX: 000664 |
| Mouse: B6.SJL-Ptprca Pepcb/BoyJ |
The Jackson Laboratory |
JAX: 002014 |
| Oligonucleotides |
|
|
| gRNA |
IDT |
N/A |
| Single strand DNA donors |
IDT |
N/A |
| Recombinant DNA |
|
|
| SOSIP expression plasmids |
This study |
N/A |
| NL4-3 ΔEnv |
NIH AIDS Reagent Program |
ARP-3418 |
| Q23-ΔEnv |
NIH AIDS Reagent Program |
ARP-11548 |
| Software and algorithms |
|
|
| Microsoft Office |
Microsoft |
https://www.office.com/
|
| GraphPad Prism v9 |
GraphPad |
N/A |
| UCSF ChimeraX |
Pettersen et al. (2021) |
https://www.cgl.ucsf.edu/chimerax/
|
| FlowJo v10.8 |
BD |
N/A |
| Illustrator |
Adobe |
N/A |
| Clustal Omega |
EMBL-EBI |
https://www.ebi.ac.uk/Tools/msa/clustalo
|
| Other |
|
|
| Protein A column |
Cytiva |
Cat# 11003493 |
| RPMI 1640 medium |
Thermo Fisher Scientific |
Cat# 61870127 |
| FBS |
Thermo Fisher Scientific |
Cat# 26140-079 |
| BSA |
Sigma-Aldrich |
Cat# A1470 |
| LS Columns |
Miltenyi Biotec |
Cat# 130-042-401 |
| Mouse B cell purification kit |
Miltenyi Biotec |
Cat# 130-090-862 |
| Mouse Pan B Cell Isolation Kit II |
Miltenyi Biotec |
Cat# 130-104-443 |
| Nucleocuvette vessels |
Lonza |
Cat# V4XP-4024 |
| P4 Primary Cell solution |
Lonza |
Cat# VSOP-4096 |
| Nucleocuvette Cartridge |
Lonza |
Cat# V4LN-7002 |
| Horse serum |
Cytiva |
Cat# SH3007403HI |
| Expi293™ Expression Medium |
Thermo Fisher Scientific |
Cat# A1435102 |
| FectoPRO transfection reagent |
Polyplus |
Cat# 116-040 |
| Desalting columns |
Thermo Fisher Scientific |
Cat# 89894 |
| Superdex 200 Increase 10/300 GL column |
Cytiva |
Cat# 28990944 |
| HiPrep 26/60 Sephacryl S400 HR column |
Cytiva |
Cat# 28935605 |
| Imject EDC mcKLH Spin Kit |
Thermo Fisher Scientific |
Cat# 77671 |
| MEGAscript® T7 Transcription Kit |
Thermo Fisher Scientific |
Cat# AMB-1334-5 |
| NanoAssembr BenchTop instrument |
Precision |
Cat# NIT0055 |
| NanoAssemblr Benchtop Acetone Cartridge |
Precision |
Cat# NIT0058 |
| Half area 96-well plate |
Corning |
Cat# 3690 |
| Globulin free BSA |
Sigma-Aldrich |
Cat# A7030 |
|
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