Figure 1. V337M mutation in MAPT: Cryo-EM cross-sections of tau filaments and immunolabelling.

a, Cross-sections through the cryo-EM reconstructions, perpendicular to the helical axis and with a projected thickness of approximately one rung, are shown for the frontal cortex from cases 1–3. Resolutions (in Å) and percentages of filament types are indicated at the bottom left and top right, respectively. Scale bar, 10 nm.

b, Immunoblotting of sarkosyl-insoluble tau from the frontal cortex of cases 1–3 with mutation V337M. Phosphorylation-independent anti-tau antibody BR134 was used.