Figure 4. R406W mutation in MAPT: Cryo-EM cross-sections of tau filaments and immunoblotting.

a, Cross-sections through the EM reconstructions, perpendicular to the helical axis and with a projected thickness of approximately one rung, are shown for temporal cortex, parietal cortex and hippocampus of case 1, and for frontal, temporal and parietal cortices of case 2. Resolutions (in Å) and percentages of filament types are indicated at the bottom left and top right, respectively. Scale bar, 10 nm.

b, Immunoblotting of sarkosyl-insoluble tau from the temporal cortex, parietal cortex and hippocampus of case 1 with mutation R406W and from frontal, temporal and parietal cortices of case 2 with mutation R406W. Phosphorylation-independent anti-tau antibody BR134 was used.