Figure 5. Tsg101^KD phenocopies a subset of evi and wg synaptic morphology and signaling defects, while loss of Hrs has no effect.

(A) Representative confocal images of muscle 6/7 NMJs labeled with α-HRP and α-BRP antibodies (left). Magnification of the yellow boxed area (right). HRP brightness was adjusted independently. Large image scale bar is 20 μm, small image scale bar is 5 μm. (B) Quantification of total bouton number (top) and active zone number (bottom) on muscle 6/7. (C) Representative confocal images of muscle 6/7 NMJ highlighting α-DLG pattern. Arrows indicate location of “feathery” DLG. Scale bar is 5 μm. (D) Representative confocal images of muscle 6/7 NMJ (abdominal segment A2) labeled with α-HRP and α-DLG antibodies. α-DLG and α-HRP signals were acquired in the linear range but adjusted independently and displayed near saturation to highlight DLG-negative ghost boutons, which are indicated with yellow arrows. (E) Quantification of baseline (i.e. unstimulated) ghost boutons. Top and bottom graphs represent independent experiments. (F) Single slices of muscle 6/7 nuclei labeled with α-LamC (nuclear periphery) and a-Fz2-C antibodies. Dotted line represents LamC-defined nuclear boundary. Scale bars are 10μm. (G) Quantification of Fz2-C puncta per nucleus. Number of nuclei quantified are indicated in the bar graph.

A2 and A3 indicate the larval abdominal segment. Data are represented as mean +/− s.e.m.; n represents nuclei in (G) and NMJs in (B-E). *p<0.05, **p<0.01, ***p<0.001. See Tables S1 and S3 for detailed genotypes and statistical analyses.