Figure 5. Evolutionary changes in overall transcriptional trajectories.

Optimal transport was used to identify “ancestors” for each cell cluster, starting with the final time point (unlike Figure 4, where transcriptomes of individual cells are measured against those of differentiated cells). These trajectories reflect the progressive divergence of transcriptomes among cells during development, and thus are an indirect reflection of cell lineages. A. Transcriptional trajectory of Lv. B. Transcriptional trajectory of He.