Figure 3. Intake and preference for EtOH during a 2-bottle choice model after adolescent binge drinking in SST-Cre:Ai9 mice.

(A) Experimental timeline. (B-E) Mice which went through adolescent DID show increased preference and intake for 3% EtOH on PND 84. (F-I) Female mice show a higher preference and intake of 7% EtOH on PND 90. (J-M) Preference and intake of 10% EtOH are highest in female mice which underwent adolescent DID. (N-Q) Sex influences both preference and intake of 10% EtOH at PND 104. (R-T) Daily EtOH intake, preference, and total fluid consumption during adulthood 2-bottle choice. (U-V) Body weight is not affected by adolescent DID. Trendlines in D-E, H-I, L-M, P-Q indicate the lines of best fit for each sex. * = p < .05, ** = p < .01, *** = p < .001, **** = p < .0001.