Table 1.
Results of GLME for all 5 adulthood drinking paradigms. Data are presented as the estimate of each fixed effect ± standard error. Significant predictors (p < .05) indicated in bold. DID: Drinking in the Dark; IA2BC: Intermittent Access 2-Bottle Choice; CIE: Chronic Intermittent Ethanol.
| Experiment 1 – Figure 2: Adolescent DID + Adulthood DID in SST-Cre:Ai9 mice | ||||
|---|---|---|---|---|
| Measurement | Sex = Male | Total Adolescent EtOH | Sex * Total Adolescent EtOH | Litter (random effect) |
| Consumption – First 2 hr Session (PND 84) | 1.822 ± 8.208 p = 0.8274 |
0.01726 ± 0.03444 p = 0.6236 |
−0.02627 ± 0.08786 p = 0.7690 |
0.0001739 |
| Consumption – First Adulthood Binge (PND 87) | −5.083 ± 4.501 p = 0.2736 |
−0.01685 ± 0.02474 p = 0.5045 |
0.03506 ± 0.047122 p = 0.4664 |
0.0001193 |
| Total Adulthood EtOH Consumption | −15.79 ± 30.93 p = 0.6156 |
0.4908 ± 0.1687 p = 0.009009 |
−0.03798 ± 0.3232 p = 0.9077 |
0.002915 |
| Experiment 2 – Figure 3: Adolescent DID + Adulthood 2BC in SST-Cre:Ai9 mice | ||||
| Measurement | Sex = Male | Total Adolescent EtOH | Sex * Total Adolescent EtOH | Litter (random effect) |
| Intake – 3% EtOH | 5.101 ± 2.8835 p = 0.1046 |
0.02655 ± 0.01756 p = 0.1587 |
−0.09984 ± 0.02935 p = 0.005905 |
1.930 |
| Preference – 3% EtOH | 3.464 ± 2.770 p = 0.2370 |
0.02169 ± 0.01895 p = 0.2769 |
−0.03723 ± 0.02845 p = 0.2173 |
0.2175 |
| Intake – 7% EtOH | −46.17 ± 10.75 p = 0.001264 |
−0.09348 ± 0.07136 p = 0.2169 |
0.3676 ± 0.1107 p = 0.006831 |
0.0009152 |
| Preference – 7% EtOH | −2.762 ± 1.093 p = 0.0281 |
−0.01321 ± 0.007305 p = 0.09774 |
0.01398 ± 0.01097 p = 0.2286 |
0.6993 |
| Intake – First 10% EtOH (PND92) | −70.17 ± 16.55 p = 0.001388 |
−0.04897 ± 0.1058 p = 0.6524 |
0.5338 ± 0.1688 p = 0.009056 |
4.557 |
| Preference – First 10% EtOH (PND92) | −3.152 ± 1.385 p = 0.04390 |
0.01466 ± 0.009742 p = 0.1606 |
0.01930 ± 0.01355 p = 0.1821 |
0.5054 |
| Intake – Last 10% EtOH (PND124) | −46.09 ± 17.67 p = 0.02432 |
−0.03719 ± 0.1162 p = 0.7550 |
0.2703 ± 0.1814 p = 0.1642 |
2.074 |
| Preference – Last 10% EtOH (PND124) | −5.968 ± 2.086 p = 0.01692 |
−0.02303 ± 0.01745 p = 0.2164 |
0.03447 ± 0.01868 p = 0.0947 |
0.7941 |
| Experiment 3 – Figure 4: Adolescent IA2BC + Adulthood IA2BC in C57BL/6J mice | ||||
| Measurement | Sex = Male | Total Adolescent EtOH | Sex * Total Adolescent EtOH | Litter (random effect) |
| Intake – First Adulthood Session (PND 90) | −26.47 ± 11.9 p = 0.04796 |
−0.05070 ± 0.03357 p = 0.1591 |
0.1063 ± 0.05705 p = 0.08939 |
Not Calculated |
| Preference – First Adulthood Session (PND 90) | −4.3317 ± 1.649 p = 0.02354 |
−0.009678 ± 0.004750 p = 0.06640 |
0.01968 ± 0.007740 p = 0.02732 |
Not Calculated |
| Intake – Last Adulthood Session (PND 120) | −21.33 ± 5.328 p = 0.002071 |
0.02280 ± 0.01503 p = 0.1574 |
0.06732 ± 0.02554 p = 0.02317 |
Not Calculated |
| Preference – Last Adulthood Session (PND 120) | −0.6270 ± 0.2718 p = 0.04155 |
0.0003290 ± 0.0007669 p = 0.6762 |
0.002752 ± 0.001303 p = 0.05843 |
Not Calculated |
| Total Adulthood Intake | −308.68 ± 75.49 p = 0.01793 |
−0.2443 ± 0.2123 p = 0.2757 |
1.029 ± 0.3619 p = 0.01595 |
Not Calculated |
| Experiment 4 – Figure 5: Adolescent IA2BC + Adulthood IA2BC in Wistar rats | ||||
| Measurement | Sex = Male | Total Adolescent EtOH | Sex * Total Adolescent EtOH | Litter (random effect) |
| Intake – First Adulthood Session (PND 70) | −1.030 ± 1.028 p = 0.3402 |
0.03604 ± 0.04408 p = 0.4326 |
0.03923 ± 0.08853 p = 0.6671 |
Not Calculated |
| Preference – First Adulthood Session (PND 70) | −1.922 ± 2.728 p = 0.4971 |
0.08718 ± 0.05318 p = 0.1322 |
0.1207 ± 0.1933 p = 0.5464 |
Not Calculated |
| Intake – Last Adulthood Session (PND 77) | 0.3826 ± 1.013 p = 0.7135 |
0.02358 ± 0.0434 p = 0.5988 |
−0.01969 ± 0.08716 p = 0.8258 |
Not Calculated |
| Preference – Last Adulthood Session (PND 77) | 1.540 ± 1.092 p = 0.1885 |
0.0213 ± 0.05213 p = 0.6914 |
−0.07011 ± 0.09279 p = 0.4673 |
Not Calculated |
| Total Adulthood Intake | −2.376 ± 4.248 p = 0.5884 |
0.3245 ± 0.1821 p = 0.1051 |
0.05722 ± 0.3657 p = 0.8788 |
Not Calculated |
| Experiment 5 – Figure 6: Adolescent CIE + Adulthood CIE in Fischer 344 rats | ||||
| Measurement | Sex = Male | Total Adolescent EtOH | Sex * Total Adolescent EtOH | Litter (random effect) |
| Intake – First Adulthood Session | 0.5628 ± 3.6244 p = 0.8817 |
0.003936 ± 0.005046 p = 0.4650 |
−0.00622 ± 0.01107 p = 0.5945 |
Not Calculated |
| Intake – Last Adulthood Session | 4.479 ± 4.062 p = 0.3124 |
0.01035 ± 0.005654 p = 0.1170 |
−0.01533 ± 0.01240 p = 0.2626 |
Not Calculated |
| Total Adulthood Intake | −77.95 ± 80.90 p = 0.3725 |
0.2103 ± 0.1126 p = 0.1111 |
0.09023 ± 0.2470 p = 0.7275 |
Not Calculated |
Models for Experiments 1 and 2: [outcome variable] ~ 1 + Sex*Total Adolescent EtOH + (1|litter)
Models for Experiments 3, 4, 5: [outcome variable] ~ 1 + Sex*Total Adolescent EtOH