FIG. 2.

Summary of the positions of HMG I binding sites within the 5′-LTR. Shown is the sequence of the 5′-LTR from −187 to +230. Previously identified transcription factor binding sites are also shown (2, 44). Solid and hatched rectangles below the sequence represent high- and low-affinity binding sites for HMG I, respectively. The shaded region (+10 to +155) indicates the approximate location of the positioned nucleosome identified by in vivo footprinting (43). The three AP-1 binding sites within this nucleosome are labeled AP1-1, AP1-2, and AP1-3. The two T residues that were mutated to G residues in footprint region 5 are highlighted with stars. Bracketed is the DNA probe used for gel shift analysis. R, start site of transcription.