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[Preprint]. 2024 May 30:2024.05.05.592554. [Version 4] doi: 10.1101/2024.05.05.592554

Figure 4.

Figure 4.

Adrb1 signaling does not alter klf2a expression in ccm2 CRISPR embryos. Tg(klf2a:H2b-EGFP; kdrl:mcherry) embryos were injected and nuclear EGFP signal in mcherry labeled vascular endothelial cells is recorded by confocal. Representative images from each group are shown. (A and B) Neither embryos injected with ccm2 MO together with adrb1 MO (A) nor control MO (B) displayed mosaic pattern of endothelial nuclear EGFP intensity. (C through E) All the embryos are absent of blood flow due to tnnt morpholino injection. Compared to that of control (C), ccm2 CRISPR embryos co-injected with adrb1 MO (D) or control MO (E) both displayed a mosaic increase of nuclear EGFP intensity of vascular endothelial cells. Scale bar:100μm. (F) EGFP intensity of endothelial nuclei from 2 embryos in each group are quantified with Image J. Statistical analysis is performed by one-way ANOVA followed by Tukey’s multiple comparison test. There is no significant difference between ccm2 morphants coinjected with adrb1 MO (A) and control MO (B). There is also no significant difference between ccm2 CRISPR embryos coinjected with adrb1 MO (D) and control MO (E). P>0.05. (F) At 2dpf, 2,3-BDM prevented the CVP cavernoma dramatically. 164 embryos in 2,3-BDM treated group and 177 in control group were used for Two-tailed paired t-test. P=0.0013.