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[Preprint]. 2024 Apr 24:rs.3.rs-4289147. [Version 1] doi: 10.21203/rs.3.rs-4289147/v1

Figure 2. Gut microbial depletion reduces oligodendrocyte lineage cell proliferation after TBI.

Figure 2

a Experimental design: one week of VNAM treatment after TBI; animals were sacrificed one week after injury. Representative images of CC (white dashed lines) stained with b dMBP, c Olig1+/BrdU+, and d bAPP. e Quantification of percentage of the dMBP-stained area of the CC. f Quantification of Olig1+/BrdU+ cells in the ipsilateral CC. g Quantification of bAPP axonal swelling density of the CC. h Experimental design: Germ free (GF) mice were gavaged with two fecal microbiota transplants (FMT) of the gut microbiota from mice treated with VNAM or Kool-Aid uninjured animals on Day -7 and day -17 prior to injury. i Representative fluorescent images of CC (white dashed lines) stained with dMBP. j Quantification of percentage of the dMBP-stained area of the CC. Mean values are plotted ± SEM. *p<0.05. Unpaired t tests was used to determine statistical differences; n=5–9 mice per group. Scale bar=200 μm and 50 μm in the inserts. Abbreviations: CC: corpus callosum; CCI: controlled cortical impact; CX: cortex; dMBP: degraded myelin basic protein; FMT: fecal matter transplant; GF: germ free; SPF: specific pathogens free; VNAM: vancomycin, neomycin-sulfate, ampicillin, and metronidazole.