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[Preprint]. 2024 Apr 26:rs.3.rs-4259413. [Version 1] doi: 10.21203/rs.3.rs-4259413/v1

Figure 6. Expression of mitochondrial thioredoxin family enzymes in FRDA mouse and cell models.

Figure 6

SDS-PAGE/immunoblot of (A) cerebellum and (C) heart lysates from wild-type and FRDAkd mice treated with (+) or without (−) doxycycline water for 18 weeks. Densitometry data from (B) cerebellum and (D) heart are expressed as mean ± standard deviation depicting 4 female (circle) and male (square) mouse subjects analyzed by one-way ANOVA. (E) SDS-PAGE/immunoblot and densitometry of lysates from dermal fibroblasts isolated from control and FRDA patients. (F) Densitometry data from patient fibroblasts expressed as mean ± standard deviation of 5–7 cell lines depicting female (circle) and male (square) subjects analyzed by a student’s t test. Lysates were probed with antibodies against FXN, Prdx3, Prdx5, Trx2, and TrxR2 with β-actin as a loading control. Statistical significance is defined as *p<0.05, **p<0.01, p<0.001 and ††p<0.0001.

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