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Effect of PLC inhibitor U73122 on Tat-induced production of IL-10. Monocytes (106) were treated or not with U73122 (2.5 or 7.5 μM) for 30 min. Tat (10 nM) was then added for 24 h. Culture supernatants were recovered, and the presence of IL-10 was determined by ELISA. The values are the means ± SD of three experiments. Similar results were obtained with cells from three different donors.
