FIG. 8.

Role of NF-κB in Tat-induced IL-10 production. (A) Effect of the serine protease inhibitor TLCK (50 and 100 μM) on NF-κB nuclear translocation induced by Tat (10 nM) (lanes 7 and 8). Lanes 3 and 4 correspond to the activation of NF-κB with Tat (10 and 1 nM, respectively). Ctr, control. (B) Effect of treatment with TLCK (TL) at 50 and 100 μM on IL-10 production by monocytes (10⁶) treated with 10 nM Tat (T). The values are the means ± SD of three experiments. Similar results were obtained with cells from three different donors.