Fig. 6. Identification of membrane proteins containing a TBC1D23-binding acidic TLY motif.

(A) Schematic of four human proteins that have an acidic TLY motif in their cytoplasmic tail and that have been reported to recycle from endosomes to the Golgi or be localized to the Golgi (SP, signal peptide; TMD transmembrane domain). (B) Binding affinities of the indicated peptides containing the acidic TLY motifs from the proteins shown in (A), as determined by ITC (fig. S5; source data are in data S2.). (C) Coomassie-stained gel showing the binding of the N-terminal cytoplasmic tail of TVP23C [His₆-MBP-TVP23C (1 to 34)] to beads coated with the C-terminal domain of TBC1D23 fused to GST. Mutations in the acidic TLY motif disrupt the interaction. Representative of two repeats.