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Growth curves of plaque-purified molecularly cloned viruses. Plaque-purified wild-type TGEV and recombinant TGEV viruses (icTGEV-1, icTGEV-2, and icTGEV-3) derived from the infectious construct were inoculated into ST cells at an MOI of 5 for 1 h at room temperature. The virus was removed, and the cultures were incubated in complete medium at 37°C. Samples were harvested at the indicated times and assayed by plaque assay in ST cells.
