Figure 4.

Radioprotective effects of BML-277 in hPBMCs. (A) hPBMCs were treated with or without BML-277 (2.5 µM) for 24 h following 1 Gy of radiation. The cells were analyzed for Annexin V binding and for PI uptake using flow cytometry. Representative bar graphs show the mean ± SEM of three independent experiments; ^*P<0.05 and ^**P<0.01 compared with the control. ⁺P<0.05 vs. the irradiated control. (B) The expression of ATM, CHK2, p53 and H2AX phosphorylation were evaluated using western blotting. IR, ionizing radiation; hPBMCs, human peripheral blood mononuclear cells;ATM, ataxia telangiectasia mutated; CHK2, checkpoint kinase 2; H2AX, H2A histone family member X.