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. 2024 Mar 21;134(10):e174587. doi: 10.1172/JCI174587

Figure 7. R77Q mutation promotes AARS1 activity and GC cell growth.

Figure 7

(A) AARS1 mutations in patients with GC from COSMIC and cBioPortal databases. (B) Structural view of R77, R77Q, and lactate in the catalytic core of AARS1. (C) In vitro lactylation assay to assess the catalytic efficiencies of WT AARS1 and R77Q mutant AARS1. (D) Immunoblot analysis of YAP lactylation in HEK293FT cells cotransfected with FLAG-YAP and WT or R77Q mutant AARS1. (E) Real-time qPCR analysis of CTGF and CYR61 mRNA levels in HEK293FT cells cotransfected with FLAG-YAP and WT AARS1 or R77Q mutant AARS1 treated with or without lactate (n = 3). Data are presented as mean ± SD. (F and G) Cell growth curves (F) and colony formation assay (G) of WT AARS1– and R77Q mutant–overexpressing AGS cells (n = 3). Data are presented as mean ± SD. Unpaired 2-tailed Student’s t test (E); 1-way ANOVA (F).