FIG. 2.
(A) SDS-PAGE of [35S]methionine-labeled cell lysate of individual EAV-VRP-infected BHK-21 cells (identified as GL, M, and GL/M). (B) Immunoblotting of recombinant proteins and EAV with various GL and M protein-specific antibodies. Antigens in each lane are indicated above the lanes. Lysate of uninfected BHK-21 cells was used as the negative control antigen (CT). Antibody (IgG) used in each lane is indicated above the antigens. The proteins were either treated with glyco F (+) or mock treated (−), as indicated below the lanes. Proteins were also analyzed by SDS-PAGE under reducing (with 50 mM DTT [+]) and nonreducing (without 50 mM DTT [−]) conditions. The proteins and their Mrs are indicated on either side of each panel.