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. 2024 Mar 19;134(10):e169137. doi: 10.1172/JCI169137

Figure 1. Increased histamine-induced vascular leakage in ISCLS skin.

Figure 1

(A) 3D rendering of fibrinogen (green) and CD31 (red) immunostaining in representative skin biopsies obtained after intradermal challenge with saline or histamine. Scale bar: 10 μm. Original magnification, ×63. (B) Ratio of fibrinogen/CD31 area (μm2). Data indicate the mean ± SEM. n = 7 ISCLS; n = 5 controls (CTRL). ***P = 0.0006, by 2-way ANOVA with Šidák’s multiple comparisons. (C and D) α-Smooth muscle actin (SMAA, green) or CD31 (red) immunostaining (left panels) and 3D rendering (right panels) in representative skin biopsies (C) and quantification of the SMAA/CD31 ratio (D). Data indicate the mean ± SEM. n = 4 ISCLS; n = 7 controls. NS, by unpaired, 2-tailed Student’s t test. Scale bar: 10 μm. Original magnification, ×63. (E and F) Collagen type IV (green) or CD31 (red) immunostaining in representative skin biopsies (E) and quantification of the collagen IV/CD31 ratio (F). Data indicate the mean ± SEM. n = 4 ISCLS; n = 7 controls. NS, by unpaired, 2-tailed Student’s t test. Scale bar: 10 μm. Original magnification, ×63.