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. 2024 Mar 19;134(10):e169137. doi: 10.1172/JCI169137

Figure 4. Structural correlates of impaired barrier function in BOECs.

Figure 4

(A) Representative images of BOECs at homeostasis (nontreated [NT]) or stimulated with histamine (20 μM) or VEGF (100 ng/mL) for 15 minutes and then stained with VE-cadherin antibody (green), phalloidin (red), or DAPI (blue). Arrowheads indicate areas of membrane disruption. Scale bar: 15 μm. Original magnification, ×63. (B) Quantification of membrane disruption as a percentage of the cell perimeter. Data indicate the mean ± SEM. n = 2–3 donors/group analyzed in 3–5 independent experiments. *P < 0.04, by2-way ANOVA with Šidák’s multiple comparisons.