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. 2024 Mar 19;134(10):e169137. doi: 10.1172/JCI169137

Figure 6. Effect of eNOS blockade in a mouse model of ISCLS.

Figure 6

(A) Analysis of histamine-induced systemic vascular leakage in SJL/J mice. (B) Representative images of EB extravasation in the legs of mice pretreated with l-NAME (10 mg/kg) or PBS and challenged with histamine (2.5 mg/kg i.p.). (C and D) Relative EB quantities in organs (C) or serum (D) from SJL/J mice challenged with histamine systemically. Data indicate the mean ± SEM. n = 3–5 mice/group. *P = 0.04 and ***P = 0.001, by 2-way ANOVA with Šidák’s multiple comparisons (C); NS, by Mann-Whitney U test (D). (E) EB content in muscle from the respective mice pretreated or not with l-NAME and challenged with histamine systemically. Data indicate the mean ± SEM. n = 4–5 mice/group. ***P < 0.0009, by 1-way ANOVA, Tukey’s multiple comparisons. (F) Analysis of vascular leakage in skin following intradermal challenge with VEGF. (G) Representative images of EB in skin challenged as indicated. Scale bar: 5 μm. (H and I) Relative EB quantities in skin (H) and serum (I) of mice challenged with VEGF intradermally. Data indicate the mean ± SEM. n = 6–7 mice/group. *P = 0.01 and ****P < 0.0001, by 2-way ANOVA with Šidák’s multiple comparisons (H); NS, by Mann-Whitney U test (I).