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. 2024 Mar 19;134(10):e169137. doi: 10.1172/JCI169137

Figure 7. Role of AMPK in eNOS hyperphosphorylation in ISCLS-derived BOECs.

Figure 7

(A and B) Representative blot (A) and quantification of p-AMPK/AMPK in BOECs left untreated or stimulated with VEGF (100 ng/mL) (B). Data indicate the mean ± SEM. n = 3–4 donors/group. *P = 0.01, by 2-way ANOVA with Šidák’s multiple comparisons. (C and D) Representative blot (C) and quantification (D) of p-AMPK/AMPK in BOECs pretreated with STO-609 (12 μM for 6 hours) or vehicle and stimulated with VEGF (100 ng/mL) for the indicated durations. Data indicate the mean ± SEM. n = 2 donors/group analyzed in 4 independent experiments. ***P = 0.0009, by 2-way ANOVA with Šidák’s multiple comparisons. (E and F) TER (E) and AUC (F) in BOECs pretreated with vehicle or STO-609. n = 2 donors/group analyzed in 4 independent experiments. NS, by 1-sample Student’s t test. (G) Representative blot (G) and quantification (H) of p-eNOS/eNOS in BOECs left untreated or pretreated with STO-609 and stimulated with VEGF (100 ng/mL). Data indicate the mean ± SEM. n = 3–4 donors/group. NS, by 2-way ANOVA with Tukey’s multiple comparisons.