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. 2024 Feb 23;21(5):882–888. doi: 10.1038/s41592-024-02196-8

Fig. 5. Multi-sheet RESOLFT time-lapse imaging of cell division.

Fig. 5

HeLa cells labeled with H2B-rsEGFP2 were imaged every 15 min for 4 h and 45 min. a, The full FOV of the recording is shown with a red square indicating the smaller FOV shown in b and c. b, Two frames taken from a volumetric rendering of the time-lapse recording (Supplementary Video 3). c, Four selected frames from the maximum intensity projection time-lapse showing significant steps in the cell division process. All images have been reconstructed using the Simple deskew algorithm (Methods). Scale bars: a, 20 µm; c, 5 µm.