Fig. 5. HIP target library for identification of TCR with new HIP specificity.
a, Schematic of HIPs generated by the post-translational fusion between insulin and other secretory granule proteins. b, ES plot of TCR3 screen against the SABR-II HIP library. Six putative hits were selected (numbered and highlighted by the larger dots) for single SABR validation across the high (green dashed line) and low (orange dashed line) confidence zones. c, Single SABR assays of 14 amino acid HIPs spanning the fusion junctions incubated against TCR3 or TCR4 expressing Jurkat cells. Bar plots depict mean of percentage GFP+CD69+ SABR-II NFAT–GFP Jurkat cells plotted from two technical replicates (dots). d, The amino acid sequences of HIPs and their right-half source proteins for epitopes in c are indicated.