TABLE 1.
Cell type | CAR, integrin, and MHC-I contenta
|
Transducibilityb
|
|||||
---|---|---|---|---|---|---|---|
CAR | αv | β3 | β5 | MHC-I | CAVGFP | AdGFP | |
HeLa | P | P | P | P | P | +++ | +++ |
DK28Cre | P | P | +++ | +++ | |||
CHOpc | L/N | P | P | P | P | − | − |
CHO-hCAR | P | P | P | P | P | +++ | +++ |
CHO-mCAR | P | P | P | P | P | +++ | +++ |
Daudi | P | L/N | L/N | N | − | − | |
E8.1 | P | L/N | L/N | P | − | ++ | |
Ramos | P | L/N | L/N | P | − | − | |
Jurkat | P | L/N | L/N | P | ++ | ++ | |
THP-1 | L/N | L/N | L/N | P | + | − | |
Dendritic cellsc | L/N | P | L/N | P | P | − | +++ |
MRC-5 | P | P | P | P | ++ | +++ | |
GM02894 | P | P | P | P | ++ | +++ | |
M21-L12 | L/N | L/N | L/N | L/N | P | ++ | +++ |
M21-L4 | P | P | P | P | P | +++ | +++ |
CS-1 | P | P | L/N | L/N | P | +++ | +++ |
CS-1/β3 | P | P | P | L/N | P | ++ | + |
CS-1/β5 | P | P | L/N | P | P | +++ | +++ |
P, present; L/N, little or none.
+++, >40% of the cells were GFP positive, using a multiplicity of infection of 103 particles/cell; ++, 10 to 40% of the cells were GFP positive; +, 1 to 10% of the cells were GFP positive; −, ≤1% of the cells were GFP positive.
Dendritic cells express αMβ2 integrins.