FIG. 2.

Expression of wild-type and mutant Vpr. (A) Immunoblot analysis of Vpr in cells. HeLa cells were infected with vaccinia virus vTF7-3 and transfected with wild-type and mutant Vpr expression plasmids. Cell lysates were processed for immunoblot analysis as described in Materials and Methods. M, molecular mass markers (in kilodaltons). Lanes: 1, pCDNA3; 2, Vpr-FL; 3, VprΔ44-FL; 4, VprΔ42-46-FL; 5, VprΔ40-48-FL; 6, VprΔ37-50-FL; 7, VprΔ37-50-H-FL. Arrow, position of proteins. (B) Expression of Vpr harboring substitutions in HII domain in HeLa cells.