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Subcellular localization of wild-type and mutant Vpr. HeLa cells 24 h after transfection were fixed and stained with anti-Flag M2 monoclonal antibody-FITC conjugate followed by DAPI. Cells were analyzed using a confocal microscope at ×60 magnification. To produce a merged image, each fluorochrome was recorded and the superimposed images were generated with Image-Pro software. (A) Anti-Flag M2-FITC conjugate; (B) DAPI; (C) superimposed images.
