FIG. 1.

Genetic organization of the ZAM retroelement. The retrovirus-like gag, pol, and env ORFs are flanked by 5′ and 3′ LTRs. The pol and env riboprobes used for in situ hybridization experiments are shown, as are ZAM polypeptides used for preparing polyclonal antibodies directed against Env and Gag proteins (pEnv and pGag, respectively). 1 and 2, oligonucleotides used for the PCR amplification of the gag ORF. The BglII and DraI restriction sites in the env ORF are those used for subcloning the envelope-encoding region into the pRSETC expression vector.