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. 2024 Apr 10;25(5):2220–2238. doi: 10.1038/s44319-024-00128-3

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

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(A) Schematic illustrates an experimental approach to determine proteomic (quantitative mass spectrometry, qMassSpec) and phosphoproteomic (qP-Proteomics) changes in response to PP2A/PP5 inhibitor LB-100 in human colorectal adenocarcinoma (hCoAD) cell line SW-480. (B) Volcano plot shows protein expression changes in response to 4 μM LB-100 stimulation (12 h) compared to untreated controls (Untr.). Upregulated (red) and downregulated (blue) proteins with false discovery rate (FDR) <0.05 (t-test) and |Log₂FoldChange| >0.58 are shown. (C) Volcano plot shows phosphorylation changes in response to 4 μM LB-100 stimulation (12 h) compared to untreated controls. Hyper- (red) and hypophosphorylated (blue) events with FDR <0.05 (t-test) and |Log₂FC| >0.58 are shown. (D) Gene Ontology (GO) analysis for 1061 hyperphosphorylated proteins. BP biological process. (E) GO analysis for 649 hypophosphorylated proteins. (F) The Donut chart shows the distribution of proteins with significantly changed (FDR <0.05 and |Log₂FC| >0.58) phosphorylation status. (G) Interaction maps of spliceosome components hyper- and hypophosphorylated (left) in response to 12 h of LB-100 stimulation in SW-480 cells. Corresponding analysis of protein expression changes is shown (right). Connecting lines show interactions from STRINGdb. Data information: In (D, E) p values were calculated with Fisher’s exact test. In (F) ****p < 0.0001 (hypergeometric test vs. background). See also Fig. EV1 and Datasets EV1, EV2.