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. 2024 Apr 10;25(5):2220–2238. doi: 10.1038/s44319-024-00128-3

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

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Figure EV2 — (A–D) Violin plots show no effect on levels of proteins resulting from alternatively spliced transcripts in MXE (A), RI (B), alt5’ss (C), and alt3’ss (D) in 4 μM LB-100-treated SW-480 cells compared to control in n = 4 biological replicates. (E) Schematic illustrates an experimental approach to determine time-resolved transcriptomic changes of alternatively spliced mRNAs in response to 4 μM LB-100 stimulation. (F) Graphs show time-resolved changes in alternatively spliced transcripts and mRNAs from RI, MXE, alt5’ss, and alt3’ss groups in response to LB-100 stimulation. Data information: In (F), statistical significance was calculated with one-way ANOVA, **p < 0.01; *p < 0.05; n. s. not significant. Source data are available online for this figure.