Table 3.

Calibration data for different analytes.

Analyte	Concentration of calibration standards (nM)	Within runs (n = 3)			Between runs (n = 3)
		Back calculated concentration (mean ± SD)	Precision (%) RSD	Accuracy (%) RE	back calculated concentration (mean ± SD)	Precision (%) RSD	Accuracy (%) RE
SN 38	0.4	0.39 ± 0.04	8.56	104.94	0.40 ± 0.04	9.02	100.94
	0.8	0.85 ± 0.04	2.99	105.71	0.83 ± 0.07	8.05	104.17
	1	0.98 ± 0.06	7.41	98.47	1.00 ± 0.08	7.70	99.69
	2	2.06 ± 0.12	6.53	102.84	2.05 ± 0.19	9.21	102.54
	10	9.29 ± 0.62	4.76	92.92	9.82 ± 0.61	6.16	98.18
	20	19.14 ± 0.54	2.79	95.71	19.28 ± 0.85	4.39	96.42
	100	96.67 ± 1.20	1.96	96.67	96.75 ± 8.08	8.35	96.75
MTX	0.4	0.40 ± 0.05	7.99	98.77	0.41 ± 0.04	10.24	101.32
	0.8	0.74 ± 0.06	4.62	92.30	0.77 ± 0.06	7.62	96.43
	1	0.97 ± 0.04	3.08	97.09	0.99 ± 0.05	4.62	99.46
	2	2.04 ± 0.10	7.69	101.81	2.02 ± 0.14	6.69	101.09
	10	10.02 ± 0.27	0.34	100.20	10.05 ± 0.34	3.36	100.54
	20	19.60 ± 0.28	1.75	97.98	20.11 ± 0.55	2.73	100.57
	100	105.97 ± 1.40	1.85	105.97	101.23 ± 9.62	9.51	101.23
DXd	0.4	0.40 ± 0.03	5.50	98.77	0.40 ± 0.03	6.72	100.58
	0.8	0.80 ± 0.09	11.44	99.81	0.80 ± 0.07	9.04	100.53
	1	0.96 ± 0.10	8.66	95.77	0.95 ± 0.06	6.06	95.42
	2	1.94 ± 0.11	4.67	96.80	2.09 ± 0.15	7.27	104.44
	10	9.87 ± 1.07	9.39	98.66	9.97 ± 0.71	7.17	99.71
	20	19.72 ± 1.05	4.27	98.62	19.92 ± 0.68	3.42	99.62
	100	100.15 ± 3.60	4.30	100.15	98.58 ± 8.22	8.34	98.58
MMAE	0.04	0.04 ± 0.00	7.85	95.89	0.04 ± 0.00	8.83	98.42
	0.08	0.08 ± 0.01	10.85	100.47	0.08 ± 0.01	9.57	99.40
	0.1	0.10 ± 0.01	10.91	103.89	0.11 ± 0.01	9.47	105.94
	0.2	0.21 ± 0.01	2.79	103.24	0.20 ± 0.02	8.30	102.03
	0.4	0.39 ± 0.02	4.61	96.53	0.40 ± 0.02	4.28	100.36
	0.8	0.78 ± 0.02	1.53	97.59	0.80 ± 0.03	3.49	100.15
	1	0.97 ± 0.04	3.40	97.18	0.98 ± 0.04	4.57	97.77
	2	2.07 ± 0.21	9.38	103.61	2.03 ± 0.14	6.95	101.69
	10	9.82 ± 0.13	1.88	98.15	9.99 ± 0.24	2.40	99.86
	20	19.67 ± 0.18	0.73	98.35	19.76 ± 0.27	1.37	98.81
	100	98.40 ± 0.98	0.98	98.40	98.21 ± 9.98	10.16	98.21
MMAF	0.04	0.04 ± 0.00	6.18	101.39	0.04 ± 0.00	10.64	100.31
	0.08	0.07 ± 0.00	7.80	92.00	0.07 ± 0.01	7.67	93.24
	0.1	0.10 ± 0.01	10.60	104.77	0.11 ± 0.01	6.71	106.62
	0.2	0.19 ± 0.02	8.81	96.75	0.20 ± 0.02	8.29	101.49
	0.4	0.40 ± 0.02	3.39	100.61	0.40 ± 0.02	4.16	100.39
	0.8	0.79 ± 0.01	2.28	98.52	0.80 ± 0.04	4.82	99.48
	1	0.98 ± 0.04	3.41	98.17	0.98 ± 0.05	4.71	97.79
	2	2.09 ± 0.08	3.04	104.43	2.07 ± 0.12	6.03	103.44
	10	9.92 ± 0.12	0.73	99.22	10.02 ± 0.22	2.23	100.16
	20	19.96 ± 0.34	0.85	99.79	19.86 ± 0.29	1.48	99.31
	100	98.19 ± 1.61	0.95	98.19	98.42 ± 9.05	9.19	98.42
CM	0.4	0.40 ± 0.05	11.67	99.29	0.39 ± 0.04	11.04	98.38
	0.8	0.75 ± 0.05	8.68	94.35	0.80 ± 0.07	8.77	100.38
	1	1.03 ± 0.10	9.20	103.24	1.05 ± 0.08	7.52	105.36
	2	2.03 ± 0.13	4.81	101.55	2.02 ± 0.14	6.90	101.22
	4	3.88 ± 0.05	2.58	97.01	3.93 ± 0.22	5.59	98.28
	8	7.81 ± 0.16	1.50	97.57	8.06 ± 0.32	3.99	100.71
	10	10.26 ± 0.06	0.96	102.63	10.09 ± 0.61	6.06	100.92
	20	20.56 ± 0.34	0.85	102.80	20.26 ± 0.87	4.31	101.30
	100	100.41 ± 1.78	1.25	100.41	102.58 ± 4.91	4.79	102.58
	200	195.41 ± 2.99	0.88	97.71	191.79 ± 9.95	5.19	95.90
	1000	978.70 ± 12.68	1.56	97.87	997.00 ± 72.32	7.25	99.70