Table 5.
Matrix effect assessment for each analyte in different serum matrices.
| Matrix source | Analyte | Spiked concentration (nM) | Replicates (n = 3) | ||
|---|---|---|---|---|---|
| measured (mean ± SD) | Precision (%) | Accuracy (%) | |||
|
Human Serum from MyBioSource Catalog no.: MBS170604 Lot no.: 11C5346 |
SN 38 | 1 | 1.00 ± 0.08 | 7.81 | 99.64 |
| 75 | 75.55 ± 3.54 | 4.68 | 100.74 | ||
| MTX | 1 | 0.93 ± 0.03 | 3.08 | 92.64 | |
| 75 | 74.37 ± 2.45 | 3.29 | 99.16 | ||
| DXd | 1 | 1.02 ± 0.13 | 12.81 | 101.77 | |
| 75 | 78.83 ± 3.46 | 4.38 | 105.11 | ||
| MMAE | 0.12 | 0.12 ± 0.00 | 2.27 | 102.10 | |
| 75 | 70.38 ± 2.27 | 3.22 | 93.84 | ||
| MMAF | 0.12 | 0.13 ± 0.00 | 0.55 | 112.03 | |
| 75 | 73.43 ± 3.09 | 4.21 | 97.91 | ||
| CM | 1.2 | 1.35 ± 0.12 | 9.17 | 112.81 | |
| 750 | 768.63 ± 23.52 | 3.06 | 102.48 | ||
|
Human Serum from Sigma Aldrich Catalog no.: H4522 Lot no.: SLCJ 3593 |
SN 38 | 1 | 0.96 ± 0.08 | 8.80 | 96.47 |
| 75 | 73.73 ± 5.10 | 6.20 | 98.31 | ||
| MTX | 1 | 0.97 ± 0.03 | 2.92 | 97.37 | |
| 75 | 77.00 ± 3.39 | 4.40 | 102.66 | ||
| DXd | 1 | 1.05 ± 0.06 | 5.48 | 105.07 | |
| 75 | 74.58 ± 4.72 | 6.33 | 99.44 | ||
| MMAE | 0.12 | 0.11 ± 0.01 | 9.84 | 95.51 | |
| 75 | 64.51 ± 3.34 | 5.18 | 86.01 | ||
| MMAF | 0.12 | 0.12 ± 0.01 | 6.44 | 96.12 | |
| 75 | 71.77 ± 2.63 | 3.66 | 95.70 | ||
| CM | 1.2 | 1.12 ± 0.05 | 4.66 | 93.68 | |
| 750 | 705.67 ± 49.02 | 6.95 | 94.09 | ||
|
Human Serum from Sigma Aldrich Catalog no.: H4522 Lot no.: SLCK 9619 |
SN 38 | 1 | 0.97 ± 0.03 | 3.54 | 96.97 |
| 75 | 71.18 ± 1.07 | 1.51 | 94.91 | ||
| MTX | 1 | 1.04 ± 0.04 | 4.02 | 104.01 | |
| 75 | 77.07 ± 3.39 | 4.40 | 102.75 | ||
| DXd | 1 | 1.03 ± 0.11 | 10.97 | 102.73 | |
| 75 | 73.76 ± 2.49 | 3.37 | 98.35 | ||
| MMAE | 0.12 | 0.10 ± 0.01 | 11.63 | 85.37 | |
| 75 | 74.82 ± 2.58 | 3.45 | 99.76 | ||
| MMAF | 0.12 | 0.11 ± 0.00 | 3.86 | 87.64 | |
| 75 | 73.94 ± 2.20 | 2.98 | 98.59 | ||
| CM | 1.2 | 1.10 ± 0.13 | 11.46 | 91.81 | |
| 750 | 713.92 ± 16.22 | 2.27 | 95.19 | ||
|
Human Serum from Sigma Aldrich Catalog no.: H5667 Lot no.: SLCL6524 |
SN 38 | 1 | 1.02 ± 0.12 | 11.60 | 102.24 |
| 75 | 81.14 ± 4.14 | 5.10 | 108.19 | ||
| MTX | 1 | 0.87 ± 0.03 | 3.14 | 87.24 | |
| 75 | 72.67 ± 3.63 | 5.00 | 96.89 | ||
| DXd | 1 | 1.06 ± 0.08 | 7.38 | 105.95 | |
| 75 | 81.77 ± 2.80 | 3.42 | 109.02 | ||
| MMAE | 0.12 | 0.13 ± 0.01 | 5.29 | 108.46 | |
| 75 | 68.82 ± 3.27 | 4.75 | 91.76 | ||
| MMAF | 0.12 | 0.13 ± 0.00 | 3.28 | 111.14 | |
| 75 | 71.19 ± 3.30 | 4.64 | 94.91 | ||
| CM | 1.2 | 1.34 ± 0.04 | 3.19 | 111.37 | |
| 750 | 824.77 ± 39.62 | 4.80 | 109.97 | ||
|
Rat Serum from MyBioSource Catalog no.: MBS238211 Lot no.: 155575 |
SN 38 | 1 | 1.09 ± 0.06 | 5.28 | 108.78 |
| 75 | 80.45 ± 1.27 | 1.58 | 107.27 | ||
| MTX | 1 | 1.08 ± 0.04 | 3.83 | 108.21 | |
| 75 | 71.75 ± 1.76 | 2.45 | 95.67 | ||
| DXd | 1 | 1.05 ± 0.13 | 12.61 | 104.65 | |
| 75 | 78.99 ± 3.70 | 4.68 | 105.33 | ||
| MMAE | 0.12 | 0.12 ± 0.01 | 10.41 | 103.84 | |
| 75 | 73.54 ± 0.83 | 1.13 | 98.05 | ||
| MMAF | 0.12 | 0.12 ± 0.00 | 1.64 | 100.28 | |
| 75 | 73.32 ± 1.30 | 1.77 | 97.76 | ||
| CM | 1.2 | 1.30 ± 0.09 | 6.56 | 108.74 | |
| 750 | 727.86 ± 6.62 | 0.91 | 97.05 | ||