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. 2024 May 1;16:1390915. doi: 10.3389/fnagi.2024.1390915

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Copyright © 2024 Zhang, Chu, Wang, Wang, Wang, Ji, Zhang, Shi, Cui and Kang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of testosterone deficiency on mitochondrial dynamics genes and mitochondrial structure in the hippocampus of male APP/PS1 mice. (A) Relative mRNA levels of Drp1, Mfn1, and OPA1 were detected by qPCR. (B) Representative blots for Drp1, Mfn1, and OPA1; (C) relative protein levels of Drp1, Mfn1, and OPA1 were analyzed by Western blot. The complete Western blots for each sample are provided in Supplementary Data. (D) Mitochondrial ultrastructure images were examined by transmission electron microscope. (E) The abnormal mitochondria rate was calculated by the number of abnormal mitochondria/total number of mitochondria. Data are expressed as the mean ± SD (n = 6 for qPCR, n = 5 for Western blot, n = 3 for electron microscope). *P < 0.05 and **P < 0.01.