(A-E) The graphs show representative and averaged traces of the spontaneous firing from putative VTA DA neuron before, during, and after LY2817412 (1 μM) slice treatment in (A) absence, and in presence of (B) picrotoxin (100 μM) + CGP55845 (1 μM), (C) picrotoxin (100 μM), (D) CGP55845 (1 μM), or (E) naloxone (10 μM). Scale bar: 1 s. (F) Bar graphs represent the averaged maximal effects of LY2817412 on the firing rate of VTA DA neurons in the same experimental conditions. *** p < 0.001, ###
p < 0.001.